cho k1 cell line Search Results


nfκb luc cho k1 cell line  (BPS Bioscience)
90
BPS Bioscience nfκb luc cho k1 cell line
Luciferase activities in <t>NFκB-Luc/CHO-K1</t> cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, <t>NFκB</t> <t>luciferase</t> was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
Nfκb Luc Cho K1 Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/NF-%CE%BAB+Reporter+(Luc)+-+CHO-K1+Cell+Line/pmc08779233-137-2-5
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cho-k1 cells  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection cho-k1 cells
Luciferase activities in <t>NFκB-Luc/CHO-K1</t> cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, <t>NFκB</t> <t>luciferase</t> was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
Cho K1 Cells, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line/pmc07694439-96-0-6
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cells from an rxfp1 expression cell line, cho-k1 rxfp1 gs  (DiscoverX corporation)
90
DiscoverX corporation cells from an rxfp1 expression cell line, cho-k1 rxfp1 gs
Luciferase activities in <t>NFκB-Luc/CHO-K1</t> cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, <t>NFκB</t> <t>luciferase</t> was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.
Cells From An Rxfp1 Expression Cell Line, Cho K1 Rxfp1 Gs, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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cho k1 cells  (Korean Cell Line Bank)
90
Korean Cell Line Bank cho k1 cells
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cells/pmc06096998-145-0-6
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cho-k1 cells expressing κ-opr  (Multispan)
90
Multispan cho-k1 cells expressing κ-opr
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cells Expressing κ Opr, supplied by Multispan, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cho-k1 cell line  (Cellca GmbH)
90
Cellca GmbH cho-k1 cell line
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cell Line, supplied by Cellca GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line/pm36389287-70-5-8
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cho-k1 cell line - by Bioz Stars, 2026-11
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cho-k1 parental cells  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications cho-k1 parental cells
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Parental Cells, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line/us07211564-135-0-7
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cho-k1 recombinant cell line  (Charles River Laboratories)
90
Charles River Laboratories cho-k1 recombinant cell line
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Recombinant Cell Line, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+recombinant+cell+line/us11993576-254-13-21
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cho-k1 recombinant cell line - by Bioz Stars, 2026-11
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turbocelltm line cho k1  (Rentschler Biopharma SE)
90
Rentschler Biopharma SE turbocelltm line cho k1
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Turbocelltm Line Cho K1, supplied by Rentschler Biopharma SE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line/pmc11280007-56-5-23
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turbocelltm line cho k1 - by Bioz Stars, 2026-11
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cho-k1 cell line  (Nature Biotechnology)
90
Nature Biotechnology cho-k1 cell line
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cell Line, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line/pm36282005-403-5-10
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cho k1 cell line (cho/nfat-cre: aurora biosciences™  (Vertex Pharmaceuticals)
90
Vertex Pharmaceuticals cho k1 cell line (cho/nfat-cre: aurora biosciences™
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cell Line (Cho/Nfat Cre: Aurora Biosciences™, supplied by Vertex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cho+k1+cell+line++cho+nfat+cre++aurora+biosciences+/us07312086-2956-11-16
Average 90 stars, based on 1 article reviews
cho k1 cell line (cho/nfat-cre: aurora biosciences™ - by Bioz Stars, 2026-11
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cho-k1 cell line  (Crown Bioscience)
90
Crown Bioscience cho-k1 cell line
Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on <t>CHO‐K1</t> cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.
Cho K1 Cell Line, supplied by Crown Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cho+k1+cell+line/cldn18+2++cho++k1+cell+line/pmc10410885-101-26-52
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Image Search Results


Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.

Journal: Metabolites

Article Title: The Cholesterol Metabolite Cholest-5-en-3-One Alleviates Hyperglycemia and Hyperinsulinemia in Obese ( db / db ) Mice

doi: 10.3390/metabo12010026

Figure Lengend Snippet: Luciferase activities in NFκB-Luc/CHO-K1 cells. Cells were treated with or without murine TNFα (10 ng/mL in DMSO), cholesterol (100 µM in DMSO), 5-cholestenone (100 µM choles-5-en-3-one in DMSO), or 1 µM IKK2 inhibitor IV. After 7 h of incubation, NFκB luciferase was detected using the ONE-Step TM Luciferase assay system. Values are expressed as the mean ± standard error ( n = 3 wells). * Significant difference at p < 0.05 compared to DMSO (TNFα-) treatment. † Significant difference at p < 0.05 compared to DMSO (TNFα+) treatment.

Article Snippet: In the NFκB-Luc/CHO-K1 cell line (BPS Bioscience, San Diego, CA, USA), fLUC expression is controlled by the NFκB response element located upstream of the TATA promoter and is suitable for monitoring the activity of the NFκB transcription factor through luminescence readout.

Techniques: Luciferase, Incubation

Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on CHO‐K1 cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.

Journal: Advanced Science

Article Title: Multimeric Amphipathic α‐Helical Sequences for Rapid and Efficient Intracellular Protein Transport at Nanomolar Concentrations

doi: 10.1002/advs.201800240

Figure Lengend Snippet: Effect of heparan sulfate on the entry of LK‐fused eGFPs. A) Cell penetration activities of CPP‐fused eGFPs on CHO‐K1 cells after 12 h of incubation. B) Comparison of fluorescence‐(+) cells on MDA‐MB‐231 and CHO‐K1 cells after 12 h of incubation with LK‐1–eGFP and LK‐4–eGFP at 100 × 10 −9 m and 500 × 10 −9 m . C) Cell penetrating kinetics of LK‐4–eGFP on CHO‐K1 cells at various concentrations. D) Comparison of the association and dissociation between LK‐fused proteins (2.5 × 10 −6 m ) and heparin which was measured by biolayer interferometry. eGFP (20 × 10 −6 m ) was used as a control. E) Comparison of fluorescence‐(+) cells on HeLa cells by small interfering RNA (siRNA)‐based inhibition of xylosyltransferase‐I (XylT‐I) expression. Cells were transfected by XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 1 h. F) Comparison of fluorescence‐(+) cells on HEK 293 cell lines by siRNA‐based inhibition. HEK 293T and HEK293s GnTi − cells were transfected by control siRNA and XylT‐I siRNA for 48 h and then treated with LK‐4–eGFP for 0.5 h. Fluorescence‐(+) cells were analyzed by FACS. All data points are represented as the average value of three experiments ± standard deviation. (**) and (***) indicate 0.001 ≤ p < 0.01 and 0.0001 ≤ p < 0.001, respectively.

Article Snippet: CHO K1 cells were purchased from Korean Cell Line Bank (KCLB) and cultured in Ham's F12 medium containing 10% FBS at 37 °C in the presence of 5% CO 2 .

Techniques: Incubation, Comparison, Fluorescence, Control, Small Interfering RNA, Inhibition, Expressing, Transfection, Standard Deviation